surface protein cd11b antibody  (Biomol GmbH)

Journal: Biomedicines

Article Title: Targeted Accumulation of Macrophages Induced by Microbeam Irradiation in a Tissue-Dependent Manner.

doi: 10.3390/biomedicines10040735

Figure Lengend Snippet: Figure 1. Time course of macrophage infiltration into MRT beam path in the irradiated liver. (A) γH2AX staining (green fluorescent cells over DAPI) in normal liver tissue at 12, 24 and 48 h post-MRT irradiation. (B) F4/80 staining (DAB) in normal liver tissue at 12, 24 and 48 h post-MRT irradiation. Highest concentration in beam path by 48 h with higher magnification of F4/80-positive macrophages in cross-fired region shown as insert. (C) CD11b+ cell populations (NovaRED) appear- ing around central veins at 12 and 24 h post-MRT and migrating to the beam path by 48 h. (D) At 7 days post-irradiation, macrophage organization persists along the beam, while CD11b staining significantly diminishes by this time. Orange dashed lines indicate clear macrophage “stripes”. * Asterisks indicate central veins.

Article Snippet: The following primary antibodies were employed: a rabbit polyclonal antibody against pan-macrophage biomarker CD68 (1:500, ab125212, Abcam plc, Cambridge, UK) for lung carcinoma and normal ear sections; a rabbit monoclonal antibody against CD68 (EPR23917-164) (1:1600, ab283654, Abcam plc) for normal lung sections; a rat monoclonal antibody against the panmacrophage marker CD68 (FA-11) (1:2000, MCA1957, Bio-Rad Laboratories Inc., Hercules, CA, USA) for melanoma-bearing ears; goat polyclonal antibodies against the surface protein CD206 (1:50, AF2535) and the transcription factor GATA-6 (1:200, AF1700, R&D Systems, Minneapolis, MN, USA); rabbit polyclonal antibodies against the surface protein CD11b (1:3000, NB110-89474, Novus Biologicals, Centennial, CO, USA) and a DNA damage marker γH2A.X (1:200, ab11174, Abcam plc) and rat monoclonal antibodies against panmacrophage biomarker F4/80 [CI:A3-1] (1:100, ab6640, Abcam plc), the surface protein Dectin-1 [2A11] (1:100, MA5-16477 Invitrogen) and the surface protein Ly6C [ER-MP20] (1:200, MA1-81899, Invitrogen, Carlsbad, CA, USA).

Techniques: Irradiation, Staining, Concentration Assay

Journal: Biomedicines

Article Title: Targeted Accumulation of Macrophages Induced by Microbeam Irradiation in a Tissue-Dependent Manner.

doi: 10.3390/biomedicines10040735

Figure Lengend Snippet: Figure 3. DNA damage and associated macrophage response following 800 Gy, 2 days (A–C) and 7 (A’–C’) days following MRT irradiation of the normal mouse ear. γH2AX immunostaining (A,A’) (NovaRED) depicting MRT beam paths (indicated by arrows). CD11b immunostaining (B,B’) (NovaRED). CD68 immunostaining (C,C’) (NovaRED).

Article Snippet: The following primary antibodies were employed: a rabbit polyclonal antibody against pan-macrophage biomarker CD68 (1:500, ab125212, Abcam plc, Cambridge, UK) for lung carcinoma and normal ear sections; a rabbit monoclonal antibody against CD68 (EPR23917-164) (1:1600, ab283654, Abcam plc) for normal lung sections; a rat monoclonal antibody against the panmacrophage marker CD68 (FA-11) (1:2000, MCA1957, Bio-Rad Laboratories Inc., Hercules, CA, USA) for melanoma-bearing ears; goat polyclonal antibodies against the surface protein CD206 (1:50, AF2535) and the transcription factor GATA-6 (1:200, AF1700, R&D Systems, Minneapolis, MN, USA); rabbit polyclonal antibodies against the surface protein CD11b (1:3000, NB110-89474, Novus Biologicals, Centennial, CO, USA) and a DNA damage marker γH2A.X (1:200, ab11174, Abcam plc) and rat monoclonal antibodies against panmacrophage biomarker F4/80 [CI:A3-1] (1:100, ab6640, Abcam plc), the surface protein Dectin-1 [2A11] (1:100, MA5-16477 Invitrogen) and the surface protein Ly6C [ER-MP20] (1:200, MA1-81899, Invitrogen, Carlsbad, CA, USA).

Techniques: Irradiation, Immunostaining